Gsplit 3 genome mate pro
A fluidic multi-well device comprising fluidic pumping channels and a central modular pump and manifold. A fluidic multi-well device for culturing cells comprising an internal humidity sensor and/or a fluid moat to maintain humidity. The spillway of claim 9 comprising rings that prevent adhesion of fluid. The spillway of claim 9 wherein the conduit comprises a vertical groove along the wall and toward the bottom of the destination well, optionally comprising an undercut into wall of the destination well positioned sufficiently from the exit of the conduit to prevent back flow and syphoning. The spillway of claim 8 wherein the conduit comprises an enlarged curved area to break fluid film into drops. The spillway of claim 4 comprising a small-width and/or high aspect ratio groove at the bottom along the conduit effective to cause spontaneous capillary flow.
The spillway of claim 4 comprising a radial meniscus pinning groove around the source well. The spillway of claim 4 comprising a V-cut to minimize fluid film disruption. The spillway of claim 4 comprising a step entry geometry. A self-leveling spillway in a multi-well cell culture system, the spillways comprising a geometry that causes capillary flow across the spillway for unidirectional self-level of fluid.
The device of claim 2 wherein the membrane is connected to the pneumatic manifold.
The device of claim 1 further comprising a pneumatic manifold. Wherein the barrier membrane is at least partially flexible, such that applying a pressure to the internal channels of the second plate causes the membrane to move, thereby obstructing or clearing a portion of the fluid paths of the first plate. Two or more wells comprising a three-dimensional space in each well defined by a bottom surface and a circumferential wall and an inlet and an outlet in each well a spillway conduit positioned between the at least two wells, having defined geometries that allow unidirectional fluid connectivity from above the bottom surface of a first well to a second well a network of fluid paths providing fluid connectivity between at least two of the wells through the inlet and the outlet of each of the two wells b) a detachable second plate comprising:Ī plurality of internal channels, each with an inlet opening and an outlet opening on opposing sides of the second plate, and one or more holes on the surface of the second plate in connection with each of the internal channels and c) a barrier membrane positioned between the fluid paths of the first plate and the one or more holes on the surface of the second plate, optionally bonded to the first plate, A fluidic multiwell device with an on-board pumping system comprising: